(877) 636-4978

TRA-1-81 anti-Human Antibody, 100 µL

Be the first to review this product

The TRA-1-81 Antibody reacts with a pluripotent-stem-cell-specific antigen expressed upon the surface of human embryonal carcinoma, embryonic germ, embryonic stem, and induced pluripotent stem cells.
SKU: ST11017
$140.00
 

Order Offline:

T: 877.636.4978
E: orders@esibio.com

F: 510.648.3255

 

Description

Details

The TRA-1-81 Antibody reacts with a pluripotent-stem-cell-specific antigen expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. The expression of TRA-1-81 antigen is stage-specific; as human EC and ES/iPS cells undergo differentiation, expression of TRA-1-81 antigen is lost, making it a widely used marker to characterize human ES/iPS cells and to monitor their differentiation.

Sample Data

TRA-1-81 anti-Human Antibody


Figure A: FC analysis on H1 human ES cells at a 1:100 dilution.Red histogram represents TRA-1-81 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgM was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with TRA-1-81 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).

Details

Details

Concentration 0.5 mg/mL
Species Reactivity Human
Host Mouse Monoclonal
Clone TRA-1-81
Isotype IgM
Immunogen Human embryonal carcinoma cell line 2102Ep
Formulation Aqueous buffer, 0.09% sodium azide.
Storage and Stability Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed.
Applications Tested Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF)
Recommended Dilutions Flow Cytometry 1:100
Immunocytochemistry/Immunofluorescence 1:100
It is recommended that the antibody be titrated for optimal performance for each application.
Alternative Names Podocalyxin, MGC138240, PODXL, PCLP, PC, gp200
References Andrews, P.W., et al. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361. PMID: 6396197

Henderson, J.K., et al. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337. PMID: 12110702

Schopperle, W.M., et al. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730. PMID: 17124010
Technical Documents ST11017 Technical Data Sheet

Reviews

Write Your Own Review

You're reviewing: TRA-1-81 anti-Human Antibody, 100 µL

Tags

Tags

Other people marked this product with these tags:

Use spaces to separate tags. Use single quotes (') for phrases.