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TRA-1-60 (PE) anti-Human Antibody, 100 μL

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Oct4 encodes a transcription factor containing a POU homeodomain (POU5F1) that plays a key role in embryonic development and stem cell pluripotency.
SKU: ST11018
$187.00
 

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Description

Details

ESI BIO’s TRA-1-60 (PE) Antibody is sterile, sodium azide-free, non-toxic, and free of culture pathogens making it ideal for sorting live pluripotent stem cell populations. Following collection, cells stained with TRA-1-60 (PE) are able to continue in culture with no adverse effect on proliferation and differentiation potential when compared to untreated cells.

The TRA-1-60 antibody recognizes a protein expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. The epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation making it a widely used marker to characterize human ES/iPS cells and to monitor their differentiation.

Details

Details

Concentration 0.2 mg/mL
Species Reactivity Human
Host Mouse Monoclonal
Clone TRA-1-60
Isotype IgM
Immunogen Human embryonal carcinoma cell line 2102Ep
Formulation 0.2 μm filter sterilized phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/μg of the protein (<0.01 ng/μg) as determined by the LAL test. Formulation is free from bacteria, fungi, and mycoplasma.
Storage and Stability Store at 2-8°C protected from light. Stable for 6 months from date of receipt when stored as directed. Contains no preservative and therefore should be handled under aseptic conditions.
Applications Tested Flow Cytometry
Recommended Dilutions Flow Cytometry 1:50 - 1:100
Immunofluorescence 1:50 - 1:100
It is recommended that the antibody be titrated for optimal performance for each application.
Alternative Names Podocalyxin, MGC138240, PODXL, PCLP, PC, gp200
References Chin, A.C., et al. (2007) Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol 130: 320-328. PMID: 17544536.

Schopperle, W.M., et al. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730. PMID: 17124010.

Takahashi, K., et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872. PMID: 18035408.
Technical Documents

ST11018 Technical Data Sheet

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