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PEGgel Kit, 1 mL

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PEGgel is a ready to use Polyethylene (glycol) diacrylate-based (PEGDA) hydrogel; containing all the necessary components to create a simple PEGDA hydrogel.
SKU: GS450
$180.00
 

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Description

Details

PEGgel Kit is a ready to use Polyethylene (glycol) diacrylate-based (PEGDA) hydrogel; containing all the necessary components to create a simple PEGDA hydrogel. PEGgel is an emerging scaffold for tissue engineering and regenerative medicine since polymerization can occur rapidly at room temperature and requires low energy input, has high water content, is elastic, and can be customized to include a variety of biological molecules. PEGDA is a synthetic, hydrophilic starting material which forms hydrogels in the presence of photoinitiator and UV light. PEGDA hydrogels are easily customizable since ECM proteins and/or growth factors can be incorporated into a hydrogel and its stiffness can be modulated from 10-100 kPa. PEGDA is widely recognized as biocompatible, non-immunogenic, and capable of chemical manipulation to incorporate attachment peptides, degradable peptides, and other moieties.

Details

Details

Storage and Stability Store PEGDA and PEGcure in original vials at -20 °C for up to one year.
Reconstituted solutions can be stored at -20 °C for ~ one month.
Size 1 mL
Applications Regular and 3-D cell culture, Tissue engineering, Photolithography
Technical Documents Technical Data Sheet
References Salinas CN and Anseth KS, The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities. Biomaterials. (2008) 29:2370-7.

Kloxin AM, et al.Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties Science (2009) 324, 59.

DeForest CA, Polizzotti BD, and Anseth KS, Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments Nat. Mater. (2009) 8: pp. 659-664.

Baird IS, Yau AY, and Mann BK, Mammalian cell-seeded hydrogel microarrays printed via dip-pin technology BioTech. (2008) 44:249-256.

Baek TJ et al, Photolithographic Fabrication of Poly(Ethylene Glycol) Microstructures for Hydrogel-based Microreactors and Spatially Addressed Microarrays J. Microbiol. Biotechnol. (2007) 17: 1826-1832.

Taite LJ, Rowland ML, Ruffino KA, Smith BR, Lawrence MB, West JL. Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Ann Biomed Eng. (2006) 34:1705-11.

Du Y, Lo E, Ali S, Khademhosseini A. Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs. Proc Natl Acad Sci U S A. (2008) 105:9522-7.

Khademhosseini A, Yeh J, Jon S, Eng G, Suh KY, Burdick JA, Langer R. Molded polyethylene glycol microstructures for capturing cells within microfluidic channels. Lab Chip. (2004) 4:425-30.

Liao H, Munoz-Pinto D, Qu X, Hou Y, Grunlan MA, Hahn MS. Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype. Acta Biomater. (2008) 4:1161-71.

Patel PN, Smith CK, Patrick CW Jr. Rheological and recovery properties of poly(ethylene glycol) diacrylate hydrogels and human adipose tissue.J Biomed Mater Res A. (2005) 73:313-9.

Panda P et al, Stop-Flow Lithography to Generate Cell-Laden Microgel Particles. Lab Chip (2008) 8:1056-1061.

Ma PX and Elisseeff J editors, Scaffolds in Tissue Engineering, CRC Press Boca Raton, FL 2006.

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