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DAPT, 5 mg

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Notch pathway inhibitor through γ-secretase inhibition
SKU: ST10033
$95.00
 

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Description

Details

DAPT inhibits γ-secretase complex and indirectly inhibits Notch, a γ-secretase substrate. It is known to reduce Aβ40 and Aβ42 levels in human primary neuronal cells (IC50 = 115 and 200 nM for Aβ and Aβ42 respectively) and brain extracts. Its activity causes neural cells to commit to neuronal differentiation and has been shown to reduce colony-forming efficiency in mouse neural stem cells. DAPT has also been shown to promote differentiation of pancreatic cells from human pluripotent stem cells.

Details

Details

Molecular Weight 432.46
Molecular Formula C23H26F2N2O4
Chemical Name N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine-1,1-dimethylethyl ester
CAS Number 208255-80-5
PubChem Identifier 5311272
Appearance White solid
Purity >99% by HPLC analysis
Alternative Names GSI-IX, γ-Secretase Inhibitor IX
Solubility Soluble in DMSO at 100 mM
Reconstitution For a 10 mM concentrated stock solution, reconstitute the compound by adding 1.2 mL of DMSO to the entire contents of vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes.
Storage and Stability Solid: Shipped at room temperature. Store at -20°C. Stable for 6 months when stored as directed.
Solution: Following reconstitution, store aliquots in tightly sealed vials at -20°C. Avoid repeated freeze-thaw cycles.
References Androutsellis-Theotokis, A., et al. (2006) Notch signaling regulates stem cell numbers in vitro and in vivo. Nature 442: 823-826. PMID: 16799564.

Crawford, T.Q., et al. (2007) The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling. Dev Dyn 236: 886-892. PMID: 17295317.

Dovey, H.F., et al. (2001) Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain. J Neurochem 76: 173-181. PMID: 11145990.

D’Amour, K.A., et al. (2006) Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 24: 1392-1401. PMID: 17053790.

Technical Documents

ST10033 Technical Data Sheet

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