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BioLite™ SSEA-1 (DyLight 488) anti-Human/Mouse Antibody, 100 µL

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Detects SSEA-1 expression in live, unfixed pluripotent stem cell populations by immunofluorescent staining.
SKU: ST11006
$270.00
 

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Description

Details

BioLite™ surface markers allow for direct immunofluorescent staining of live, unfixed pluripotent stem cell populations. The antibodies undergo meticulous application testing and validation to ensure they are sterile, non-toxic, contain no sodium azide, and are free of culture pathogens. Colonies stained with BioLite antibodies are able to continue in culture with no adverse effect on proliferation and differentiation potential when compared to untreated cells.

Background Stage-specific embryonic antigen-1 (SSEA-1) is a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma (EC), murine embryonic stem (ES), and murine and human germ (EG) cells. No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.

Details

Details

Concentration 0.5 mg/mL
Species Reactivity Human, Mouse
Host Mouse Monoclonal
Clone MC-480
Isotype IgM, κ
Immunogen F9 tetracarcinoma stem cells (X-irradiated)
Formulation 0.2 μm filter sterilized phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/μg of the protein (<0.01 ng/μg of the protein) as determined by the LAL test. Formulation is free from bacteria, fungi, and mycoplasma.
Storage and Stability Store at 2-8°C protected from light. Stable for 6 months from date of receipt when stored as directed.
The BioLite format contains no preservative and therefore must be handled under aseptic conditions.
Applications Tested Immunofluorescence (IF) on live, unfixed mouse ES cells
Recommended Dilutions Immunofluorescence 1:100
Flow Cytometry 1:100
It is recommended that the antibody be titrated for optimal performance for each application.
Alternative Names CD15, Stage-specific embryonic antigen 1
References Brambrink, T., et al. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159. PMID: 18371436

Draper, J.S., et al. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat 200: 249-258. PMID: 12033729
Technical Documents

ST11006 Technical Data Sheet
Protocol: Immunofluorescent Staining of Live Cells

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