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SSEA-4 anti-Human Antibody, 100 µL

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The SSEA-4 Antibody recognizes a protein expressed upon the surface of human teratocarcinoma stem, embryonic germ, embryonic stem, and induced pluripotent stem cells.
SKU: ST11015

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This antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4), a glycolipid carbohydrate epitope that is expressed upon the surface of human teratocarcinoma stem (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. No immunoreactivity is evident with undifferentiated murine EC, ES/iPS and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES/iPS cells may be accompanied by an increase in SSEA-4 expression.

Sample Data

antibody st11015

Figure A: FC analysis on H1 human ES cells at a 1:100 dilution.
Red histogram represents SSEA-4 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgG was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with SSEA-4 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).



Concentration 0.5 mg/mL
Species Reactivity Human
Host Mouse Monoclonal
Clone MC-831-70
Isotype IgG3
Immunogen Human embryonal carcinoma cell line 2102Ep
Formulation Aqueous buffer, 0.09% sodium azide.
Storage and Stability Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed.
Applications Tested Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF)
Recommended Dilutions Flow Cytometry 1:100
Immunocytochemistry/Immunofluorescence 1:100
It is recommended that the antibody be titrated for optimal performance for each application.
Alternative Names Stage-specific embryonic antigen 4
References Battula, V.L., et al. (2007) Human placenta and bone marrow derived MSC cultured in serum-free, b-FGF-containing medium express cell surface frizzled-9 and SSEA-4 and give rise to multilineage differentiation. PMID: 17288545

Kannagi, R., et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J 2: 2355-2361. PMID: 6141938
Technical Documents ST11015 Technical Data Sheet


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