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TRA-1-60 anti-Human Antibody, 100 µL

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The TRA-1-60 Antibody recognizes a protein expressed upon the surface of undifferentiated human embryonal carcinoma, embryonic germ, embryonic stem, and induced pluripotent stem cells.
SKU: ST11016
$140.00
 

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Description

Details

The TRA-1-60 antibody recognizes a protein expressed on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells. The epitope recognized by the TRA-1-60 antibody is lost upon cell differentiation making it a widely used marker to characterize human ES/iPS cells and to monitor their differentiation.

Sample Data

TRA-1-60 anti-Human Antibody


Figure A: FC analysis on H1 human ES cells at a 1:100 dilution. Red histogram represents TRA-1-60 Antibody and open histogram represents isotype control. A PE-conjugated anti-Mouse IgM was used as the secondary antibody. Figure B: ICC analysis on H1 human ES cells. Cells were stained with TRA-1-60 Antibody at a 1:100 dilution followed by an Alexa 488-conjugated secondary antibody (green). Nuclei were counterstained with DAPI (blue).

Details

Details

Concentration 0.5 mg/mL
Species Reactivity Human
Host Mouse Monoclonal
Clone TRA-1-60
Isotype IgM
Immunogen Human embryonal carcinoma cell line 2102Ep
Formulation Aqueous buffer, 0.09% sodium azide.
Storage and Stability Store at 2-8°C. Stable for 6 months from date of receipt when stored as directed.
Applications Tested Flow Cytometry (FC), Immunocytochemistry/Immunofluorescence (ICC/IF)
Recommended Dilutions Flow Cytometry 1:100
Immunocytochemistry/Immunofluorescence 1:100
It is recommended that the antibody be titrated for optimal performance for each application.
Alternative Names Podocalyxin, MGC138240, PODXL, PCLP, PC, gp200
References Chin, A.C., et al. (2007) Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol 130: 320-328. PMID: 17544536

Schopperle, W.M., et al. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730. PMID: 17124010

Takahashi, K., et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872. PMID: 18035408
Technical Documents ST11016 Technical Data Sheet

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